Lawrence Livermore National Laboratory

The following selected publications illustrate the range of applications and technology developments the resource is undertaking.

AMS Measurement

Ognibene TJ, Salazar GA. (2013) Installation of hybrid ion source on the 1-MV LLNL BioAMS spectrometer. Nuclear Instruments & Methods in Physics Research, Section B-Beam Interactions with Materials & Atoms. 294: 311-314 doi: 10.1016/j.nimb.2011.11.029

Keck BD, Ognibene T, Vogel JS (2010). Analytical validation of accelerator mass spectrometry for pharmaceutical development. Bioanalysis 2(3): 469-485. doi: 10.4155/bio.10.14

Ognibene TJ, Bench G, Brown TA, Vogel JS. (2004) The LLNL accelerator mass spectrometry system for biochemical 14C-measurements. Nuclear Instruments & Methods in Physics Research, Section B-Beam Interactions with Materials & Atoms. 223–224: 12–15. doi: 10.1016/j.nimb.2004.04.006

Vogel JS, Ognibene T, Palmblad M, Reimer P (2004). Counting Statistics and Ion Interval Density in AMS. Radiocarbon 46(3): 1103-1109.

Ognibene TJ, Bench G, Brown TA, Peaslee, Vogel JS (2002) A new accelerator mass spectrometry system for 14C-quantification of biochemical samples. International Journal of Mass Spectrometry, 218 (3): 255–264. doi: 10.1016/S1387-3806(02)00734-0

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Liquid Sample AMS

Thomas AT, Stewart BJ, Ognibene TJ, Turteltaub KW, and Bench G. (2013) Directly Coupled High-Performance Liquid Chromatography− Accelerator Mass Spectrometry Measurement of Chemically Modified Protein and Peptides. Analytical Chemistry 85(7): 3644−50. doi: 10.1021/ac303609n

Thomas AT, Ognibene T, Daley P, Turteltaub K, Radousky H, and Bench G. (2011) Ultra-high efficiency moving wire combustion interface for online coupling of HPLC. Analytical Chemistry 83(24): 9413-27.
doi: 10.1021/ac202013s

Graphite Preparation for AMS Analysis

Ognibene TJ, Bench G, Vogel JS, Peaslee GF, Murov S. (2003) A High-Throughput Method for the Conversion of CO2 Obtained from Biochemical Samples to Graphite in Septa-Sealed Vials for 14C-AMS Quantification. Analytical Chemistry 75(9):2192-6. doi: j10.1021/ac026334j

Buchholz BA, Freeman SPHT, Haack KW and Vogel JS (2000) Tips and traps in the C-14 bio-AMS preparation laboratory. Nuclear Instruments & Methods in Physics Research, Section B-Beam Interactions with Materials & Atoms 172: 404-408. doi: 10.1016/S0168-583X(00)00142-7

Vogel JS (1992). Rapid Production of Graphite Without Contamination for Biomedical AMS. Radiocarbon 34(3): 344-350.

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Human Studies

McCartt, A. D., Ognibene, T. J., Bench, G., & Turteltaub, K. W. (2016). Quantifying Carbon-14 for Biology Using Cavity Ring-Down Spectroscopy. Analytical Chemistry, doi: 10.1021/acs.analchem.6b02054.

Jubert C, Mata J, Bench G, Dashwood R, Pereira C, Tracewell W, Turteltaub K, Williams D, and Bailey G. (2009) Effects of chlorophyll and chlorophyllin on low-dose aflatoxin B1 pharmacokinetics in human volunteers: A pilot study. Cancer Prevention Research 2(12): 1015-22.
doi: 10.1158/1940-6207.CAPR-09-0099

Brown K, Tompkins EM, Boocock DJ, Martin EA, Farmer PB, Turteltaub KW, Ubick E, Hemingway D, Horner-Glister E, White IN. (2007) Tamoxifen forms DNA adducts in human colon after administration of a single [14C]-labeled therapeutic dose. Cancer Research 67(14):6995-7002.
doi: 10.1158/0008-5472.CAN-07-0913

Carkeet C, Dueker SR, Lango J, Buchholz BA, Miller JW, Green R, Hammock BD, Roth JR, Anderson PJ. (2006) Human vitamin B12 absorption measurement by accelerator mass spectrometry using specifically labeled 14C-cobalamin. Proc. Natl. Acad. Sci, USA, 103 5694-5699. doi: 10.1073/pnas.0601251103

Lin YM, Dueker SR, Follett JR, Fadel JG, Arjomand A, Schneider PD, Miller JW, Green R, Buchholz BA, Vogel JS, Phair RD, and Clifford AJ. (2004) Quantitation of in vivo human folate metabolism. American Journal Of Clinical Nutrition 80: 680-691.

Lemke SL, Dueker SR, Follett JR, Lin Y, Carkeet C, Buchholz BA, Vogel JS, Clifford AJ. (2003) Absorption and retinol equivalence of beta-carotene in humans is influenced by dietary vitamin A intake. Journal of Lipid Research. 44:1591-1600. doi: 10.1194/jlr.M300116-JLR200

Buchholz BA, Fultz E, Haack KW, Vogel JS, Gilman SD, Gee S, Hammock BD, Hui X, Wester RC, Maibach HI. (1999) HPLC-AMS Measurement of Atrazine Metabolites in Human Urine after Dermal Exposure, Analytical Chemistry 71: 3519-3525. doi: 10.1021/ac990152g

Buchholz BA, Arjomand A, Dueker SR, Schneider PD, Clifford AJ, Vogel JS. (1999) Intrinsic Erythrocyte Labeling and Attomole Pharmacokinetic Tracing of 14C-Folic Acid with Accelerator Mass Spectrometry, Analytical. Biochemistry. 269: 348-352. doi: 10.1006/abio.1999.4041

Dingley KH, Curtis KD, Nowell S, Felton JS, Lang NP, Turteltaub KW (1999). DNA and protein adduct formation in the colon and blood of humans after exposure to a dietary-relevant dose of 2-amino-1-methyl-6-phenylimidazo 4,5-b pyridine. Cancer Epidemiology Biomarkers & Prevention 8(6): 507-512.

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Animal Studies

Malfatti MA, Palko HA, Kuhn EA, Turteltaub KW. (2012) Determining the Pharmacokinetics and Long-Term Biodistribution of SiO2 Nanoparticles In Vivo Using Accelerator Mass Spectrometry. Nanoletters 12(11): 5532-5538. doi: 10.1021/nl302412f

Neuwirth C, Mosesso P, Pepe G, Fiore M, Malfatti M, Turteltaub K, Dekent W, Mally A. (2012) Furan carcinogenicity: DNA binding and genotoxicity of furan in rats in vivo. Mol Nutr Food Res., 56, 1363-1374.

Buchhloz BA, Haack KW, Sporty JL, Buckpitt AR, Morin D (2010) Free flow electrophoresis separation and AMS quantitation of 14C-naphthalene-protein adducts, Nuclear Instruments and Methods in Physics Research Section B: Beam Interactions with Materials and Atoms 268: 1324-1327.
doi: 10.1016/j.nimb.2009.10.164.

Marsden DA, Jones DJL, Britton RG, Ognibene T, Ubick E, Johnson GE, Farmer PB, Brown K (2009) Dose-Response Relationships for N7-(2-Hydroxyethyl)Guanine Induced by Low-Dose [C-14]Ethylene Oxide: Evidence for a Novel Mechanism of Endogenous Adduct Formation Cancer Research 69(7):3052-9.
doi: 10.1158/0008-5472.CAN-08-4233.

Williams KE, Carver TA, Miranda JJ, Kautiainen A, Vogel JS, Dingley K, Baldwin MA, Turteltaub KW, Burlingame AL. (2002) Attomole detection of in vivo protein targets of benzene in mice: evidence for a highly reactive metabolite. Molecular and Cellular Proteomics. 11:885-95.
doi: 10.1074/mcp.M200067-MCP200

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Cellular Studies

Stewart BJ, Navid A, Kulp KS, Knaack JL, Bench G. (2013) D-Lactate production as a function of glucose metabolism in Saccharomyces cerevisiae. Yeast 30(2):81-91. DOI: 10.1002/yea.2942

Henderson PT, Li T, He M, Zhang H, Malfatti M, Gandara D, Grimminger PP, Danenberg KD, Beckett L, de Vere White RW, Turteltaub KW, Pan CX. (2011) A microdosing approach for characterizing formation and repair of carboplatin-DNA monoadducts and chemoresistance. International Journal of Cancer 129(6):1425-34. doi: 10.1002/ijc.25814.

Stewart BJ, Navid A, Turteltaub KW, Bench G. (2010) Yeast dynamic metabolic flux measurement in nutrient-rich media by HPLC and accelerator mass spectrometry. Analytical Chemistry 82(23):9812-7.
doi: 10.1021/ac102065f

Links J, Palmblad M, Ognibene T, Turteltaub K, Bench G. (2010) Quantitative metabolism using AMS: Choosing a labeled precursor. Nuclear Instruments & Methods in Physics Research, Section B-Beam Interactions with Materials & Atoms 268(7-8): 1309–12. doi: 10.1016/j.nimb.2009.10.160

Sisi Wang, Hongyong Zhang, Michael Malfatti, Ralph de Vere White, Primo Lara, Kenneth Turteltaub, Paul Henderson,Chong-xian Pan (2010). Gemcitabine causes minimal modulation of carboplatin-DNA monoadduct formation and repair in bladder cancer cells. Chemical Research in Toxicology, 23, 1653-1655.

Sporty JL, Kabir MM, Turteltaub KW, Ognibene T, Lin SJ, Bench G (2008) Single sample extraction protocol for the quantification of NAD and NADH redox states in Saccharomyces cerevisiae. Journal of Separation Science 31(18): 3202-11. doi: 10.1002/jssc.200800238

Mundt JM, Hah SS, Sumbad RA, Schramm V, Henderson PT (2008) Incorporation of extracellular 8-oxodG into DNA and RNA requires purine nucleoside phosphorylase in MCF-7 cells. Nucleic Acids Research 36(1): 228-36. doi: 10.1093/nar/gkm1032

Hah SS, Mundt JM, Kim HM, Sumbad RA, Turteltaub KW, Henderson, PT (2007) Measurement of 7,8-dihdro-8-oxo-2'deoxyguanosine metabolism In MCF-7 cells at low concentrations use accelerator mass spectrometry. Proc. Natl. Acad. Sci, USA, 104(27): 11203-11208.
doi: 10.1073/pnas.0701733104

Ting LM, Shi W, Lewandowicz A, Singh V, Mwakingwe A, Birck MR, Taylor Ringia EA, Bench G, Madrid DC, Tyler PC, Evans GB, Furneaux RH, Schramm VL, Kami, K. (2005) Targeting a Novel Plasmodium falciparum Purine Recycling Pathway with Specific Immucillins. Journal of Biological Chemistry, 280(10), 9547 – 9554.
doi: 10.1074/jbc.M412693200

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Bomb Pulse Biology

Sarachine Falso MJ, Buchholz BA. (2013) Bomb Pulse Biology. Nuclear Instruments & Methods in Physics Research, Section B-Beam Interactions with Materials & Atoms. 294:666-670.
doi: 10.1016/j.nimb.2012.08.045

Spalding KL, Bergmann O, Alkass K, Bernard S, Salehpour M, Huttner H, Boström E, Westerlund I, Buchholz BA, Possnert G, Mash D, Druid H, Frisén J. (2013) Dynamics of hippocampal neurogenesis in adult humans. Cell 153 (6): 1219-1227.
doi: 10.1016/j.cell.2013.05.002

Arner P, Bernard S, Salehpour M, Possnert G, Liebl J, Steier P, Buchholz BA, Eriksson M, Arner E, Hauner H, Skurk T, Rydén M, Frayn KN, Spalding KL. (2011) Dynamics of human adipose lipid turnover in health and metabolic disease. Nature 478:110-113.
doi: 10.1038/nature10426

Perl S, Kushner JA, Buchholz BA, Meeker AK, Stein GM, Hsieh M, Kirby M, Pechhold S, Liu EH, Harlan DM, Tisdale JF. (2010) Significant Human β-Cell Turnover Is Limited to the First Three Decades of Life as Determined by in vivo Thymidine Analog Incorporation and Radiocarbon Dating. Journal of Clinical Endocrinology and Metabolism. 95: E234-E239.
doi: 10.1210/jc.2010-0932

Bergmann O, Bhardwaj RD, Bernard S, Zdunek S, Barnabé-Heider F, Walsh S, Zupicich J, Alkass K, Buchholz BA, Druid H, Jovinge S, Frisén J. (2009) Evidence for cardiomyocyte renewal in humans. Science 324: 98-102.
doi: 10.1126/science.1164680

Spalding KL, Arner E, Westermark PO, Bernard S, Buchholz BA, Bergman OI, Blomqvist L, Hoffstedt, Näslund JE, Britton T, Concha H, Hassan M, Rydén M, Frisén J, Arner P. (2008) Dynamics of Fat Cell Turnover in Humans. Nature 453 : 783-787. doi: 10.1038/nature06902

Spalding KL, Bhardwaj RD,Buchholz BA, Druid H, Frisén J. (2005) Retrospective Birth Dating of Cells. Cell 122:133-143.
doi: 10.1016/j.cell.2005.04.028

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Forensic Medicine

Alkass K, Saitoh H, Buchholz BA, Bernard S, Holmlund G, Senn DR, Spalding KL, Druid H. (2013) Analysis of radiocarbon, stable isotopes and DNA in teeth to facilitate identification of unknown decedents. PLoS ONE 8(7): e69597.
doi: 10.1371/journal.pone.0069597

Spalding KL, Buchholz BA, Druid H, Bergman L, Frisén J. (2005) Forensic Medicine: Age Written in Teeth by Nuclear Bomb Tests. Nature 437: 333-334.
doi: 10.1038/437333a

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Enright HA and Malfatti MA. (2014) Detection Methods for the In Vivo Biodistribution of Iron Oxide and Silica Nanoparticles: Effects of Size, Surface Chemistry, and Shape, in Monteiro-Riviere NA and Tran CL., eds., Nanotoxicology: Progress Toward Nanomedicine, CRC Press, Boca Raton, p. 177-200.

Stewart BJ, Bench G, Buchholz BA, Haack KW, Malfatti MA, Ognibene TJ, Turteltaub KW. (2012). Accelerator Mass Spectrometry in Pharmaceutical Development. In: Mass Spectroscopy Handbook, Pharmaceutical Science and Biotechnology Practices, Applications and Methods series, pp 259-269 (Lee, M.S. Ed), John Wiley and Sons: Hoboken, New Jersey.
ISBN9780470536735, doi: 10.1002/9781118180730.ch12

Buchholz BA, Sarachine Falso MJ, Stewart BJ, Haack KW, Ognibene TJ, Bench G, Salazar Qunitero GA, Malfatti MA, Kulp KS, Turteltaub KW. (2012). Bioanalytics for Human Microdosing. In: Encyclopedia of Drug Metabolism and Interactions, Vol. 5, chapter 21, pp 1-28 (Lyubimov, A.V. Ed), John Wiley and Sons, (2012), Chapter 21.
ISBN: 978-0-470-45015-4, doi: 10.1002/9780470921920.edm112

Vogel JS, Love AH (2005) Quantitating Isotopic Molecular Labels with Accelerator Mass Spectrometry. Methods in Enzymology 402: 402-422.
doi: 10.1016/S0076-6879(05)02013-6

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