Chemotherapy helps save the lives of thousands of cancer patients each year. But now, researchers at the Lawrence Livermore Lab and UC Davis believe they've developed a technology that could make chemo treatments even more effective. Channel 7 (KGO-TV, San Francisco) aired this story about the LLNL-UC Davis cancer collaboration.
LLNL Scientists' paper published in Analytical Chemistry highlights the use of Liquid sample AMS
"Directly Coupled High-Performance Liquid Chromatography–Accelerator Mass Spectrometry Measurement of Chemically Modified Protein and Peptides" Avi T. Thomas, Benjamin J. Stewart , Ted J. Ognibene , Kenneth W. Turteltaub , and Graham Bench
Quantitation of low-abundance protein modifications involves significant analytical challenges, especially in biologically important applications, such as studying the role of post-translational modifications in biology and measurement of the effects of reactive drug metabolites. 14C labeling combined with accelerator mass spectrometry (AMS) provides exquisite sensitivity for such experiments. Here, we demonstrate real-time 14C quantitation of high-performance liquid chromatography (HPLC) separations by liquid sample accelerator mass spectrometry (LS-AMS). By enabling direct HPLC–AMS coupling, LS-AMS overcomes several major limitations of conventional HPLC–AMS, where individual HPLC fractions must be collected and converted to graphite before measurement."
Collaboration with Swedish Scientists uses bomb pulse 14C to reveal dynamics of human hippocampal neurogenesis
"Dynamics of Hippocampal Neurogenesis in Adult Humans" Kirsty L. Spalding, Olaf Bergmann, Kanar Alkass, Samuel Bernard, Mehran Salehpour, Hagen B. Huttner, Emil Boström, Isabelle Westerlund, Céline Vial, Bruce A. Buchholz, Göran Possnert, Deborah C. Mash, Henrik Druid, Jonas Frisén
Adult-born hippocampal neurons are important for cognitive plasticity in rodents. There is evidence for hippocampal neurogenesis in adult humans, although whether its extent is sufficient to have functional significance has been questioned. The generation of hippocampal cells in humans has been determined by measuring the concentration of nuclear-bomb-test-derived 14C in genomic DNA, It was found that a large subpopulation of hippocampal neurons constituting one-third of the neurons is subject to exchange. In adult humans, 700 new neurons are added in each hippocampus per day, corresponding to an annual turnover of 1.75% of the neurons within the renewing fraction, with a modest decline during aging. It appears that neurons are generated throughout adulthood and that the rates are comparable in middle-aged humans and mice, suggesting that adult hippocampal neurogenesis may contribute to human brain function.