LLNL is a National Laboratory organized to facilitate multidisciplinary sharing of facilities and equipment among its staff. Resource facilities at LLNL include laboratories for sample preparation, chemical separations, physical analysis, and cell culture.
The following equipment is used by the Resource:
- We have situated a National Electrostatics Corporation 250kV accelerator mass spectrometer in a biomedical laboratory. This spectrometer represents the latest evolution in AMS technology. This high voltage power deck will afford easier and less expensive operation, with a much smaller footprint over traditional AMS systems. While high voltage decks have been validated for use with samples introduced as graphite, we aim to be the first to demonstrate and validate their use for the analysis of biomedical samples introduced as CO2 gas. Gas measurement capability is paramount to the success of producing real time high-density AMS data sets as the analysis of liquid samples necessary to achieve high throughput analysis requires their conversion to CO2. This system will be configured to take advantage of our new liquid sample AMS instrumentation to provide directly-coupled liquid sample AMS measurement. Importantly, as this system is situated in a biological/clinical setting that will be configured as a BSL-2 laboratory it will be capable of analyzing samples that are considered Risk Group 2, such as human plasma and urine samples.
National Electrostatics Corporation 250kV accelerator mass spectrometer
in a biomedical laboratory
- Another spectrometer is available to the Resource through CAMS: a 10-MV FN tandem accelerator capable of terminal voltages up to 10 MV with AMS measurement capabilities developed for 3H, 10Be, 14C, 26Al, 36Cl, 41Ca, 129I and 239Pu. A 1.7 MV tandem proton accelerator for nuclear microscopy that can measure the masses of microgram to picogram quantities of materials is also situated at CAMS.
- All animal work conducted at LLNL is carried out in an AAALAC accredited Animal Facility in accordance with Animal Welfare Assurance number A3184-01. The AAALAC accredited Animal Facility is located in a single-story unit with 9,390 square feet of usable space inclusive of supporting area and is capable of housing 8,000 to 10,000 small animals (mice, rats guinea pigs), annually. Three Laboratory Animal Technologists are responsible for animal care. Eight rooms are used for conventional housing and two rooms are used for quarantine and isolation. One room is provided with a Micro-Isolator housing system (Lab Protocols, Inc.) capable of housing 112 mice exposed to toxic agents. The air passing through these units is HEPA filtered. Another room has a Cesium-137 Irradiator capable of irradiating small objects and animals within a shielded, interlocked enclosure. Animals are under the supervision of a board-certified Veterinarian and are monitored daily for distress.
- A Waters Acquity UPLC system which includes a system controller, solvent pumps capable of 4 solvent separations, an autosampler, and a photodiode array detector, coupled to a Waters fraction collector. The system is connected to a Lenova PC equipped with Waters Empower Software .
- A Thermo LC/MS system composed of a Thermo Ultimate 3000 UHPLC system consisting of two RS pumps, an autosampler, and a photodiode array detector coupled to a Thermo LTQ XL linear ion trap mass spectrometer. The system is PC controlled with Xcalibur software.
- A PC driven Waters Alliance HPLC system consisting of a Waters 2690 separations module with auto sampler, and a Waters 996 photodiode array detector coupled to a Foxy R2 fraction collector. The system is controlled with Waters Millennium Software.
- Radioisotope labeling of mammalian cells is performed in a 200 sq ft BSL2 laboratory equipped with a class 2 biosafety cabinet, a -20° refrigerator, New Brunswick Galaxy 48R a water jacketed incubator, an Eppendorf 5804R table top centrifuge, and a VWR Vista Vision inverted microscope.
- Our biological labs contain a Perkin Elmer Tri-Carb 2910TR liquid scintillation counter, a Thermo Sorvall MTX150 micro-ultracentrifuge, a Shimadzu UV-1700 uv-visible spectrophotometer, and a Labconco centrivap console concentrator. Also present are general laboratory glassware, pipettes, American temperature controlled shaking water bath, Fisher Scientific sonicating water bath, Eppendorf micro centrifuge, Sartorius analytical balance, to vortex mixeers, a -80 °C and -20°C Freezer, a refrigerator, and fume hood. Equipment for cell culture includes a Forma Scientific water-jacketed CO2 incubator, Baker laminar flow HEPA filtered SterileGard BSL2 cabinet, Eppendorf 5415D centrifuge, Wild inverted microscope, and Ziess microscope. There are also two VWR digital heat blocks, three vortex mixers, two micro-centrifuges, a Sartorius scale, a Branson sonifier, a Nanopure Ultra Pure water system, one Thermolyne hot plate, two Corning stir/hot plates, one LaPine stir plate, one American Scientific scale, an Orion digital pH meter, and a Boekel Grant shaking water bath. There is also a PC-driven Agilent HPLC, which includes, fluorescent and diode array detectors, thermostated column guard, auto sampler, binary pump, degasser, ALS thermostat, and HP laser jet printer. Also present is a Sorvall RC 5C Plus centrifuge, one mini-beadbeater homogenizer, a Reliable Scientific plate rocker, two Eppendorf 5417R refrigerated microcentrifuge, Eppendorf Thermomixer 5436, 2 electronic pipettes, a Lauda water bath, a Biorad fraction collector, a Biorad rotofor cell, Biorad power pacs, 1000 and 3000, one Biorad Protean IEF cell, a Christ Alpha 1-2 lyophylizer and Varian pump, one Brinkmann pump, a Hamilton fume hood, one PC-driven Nanodrop, one VWR Low Temperature Incubator, a Revco –80 freezer, a Revco –20 freezer, two freezers, and two refrigerator/freezers.
- Conversion of radiocarbon-tagged biologicals to filamentous graphite is accomplished in a dedicated graphite laboratory. Present in the graphite laboratory are two Jouan RC 10.10 vacuum concentrators/dryers, one PC-controlled Exeter 440 CHN Analyzer, two Uniquip Unijet II refrigerated aspirators, four Neytech muffle furnaces, on e VWR Vacuum oven, one glove box, one Hamilton Fume Hood, three Varian Minuteman V70LP Turbopumps, two single position CO2 transfer manifolds, one quadruple position tritium gas transfer manifold, two 67 position graphitization furnaces, and a Macintosh workstation.
- Also available are automated oligonucleotide and peptide synthesizers (each with dedicated HPLC purifications systems), a PE Biosystems MALDI-TOF Mass Spectrometer, two LC-MS systems, FAB-, CI- and EI- mass spectrometers, and 300 MHz and 500 MHz NMR instrumentation.